GeneTool FAQ

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Does GeneTool operate differently in Windows and Macintosh environments?
All our software is cross platform compatible. This means that the programs operate with the same look and feel, whether being run on either a PC or Mac. 

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What is the difference between the demo version of GeneTool and the full version of GeneTool?
The demo version cannot print or save files. 

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My demo key code does not allow me access to the program.
Check the following:

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Do I have to install the demo on my hard drive to access the program?
Yes, the demo must be installed onto your hard drive. To avoid any potential system errors while running the demo after installation, please remove the CD from the drive. 

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During installation of the demo, do I have to use the default directories?
No, you may change the destination directory.

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I am trying to run GeneTool but it fails to install.
If you have downloaded the GeneTool demo but the file is called demo.cgi, rename the file to GeneTool-Demo.exe on Windows, GeneTool-Demo.sea.hqx on Mac, or GeneTool-Demo.tar.Z on UNIX. 

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What should I do when an error message appears on my screen?
Click on the Save Report button to save the error report. Enter a full path for the directory to which you would like to save the file. For example C:\error.txt. Email the error report to biotools-support@poker-academy.com as a file attachment. 

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How do I increase the number of network users?
Call a BioTools Sales Representative with the new, additional users you wish to add to your network installation. BTI will issue you with a new key to reflect the total number of concurrent network users. You will be invoiced for the additional users. A sales representative may be reached at biotools-support@poker-academy.com or by telephone at 780.423.1133. 

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How do I transfer information from GeneTool to PepTool?
If you have a copy of PepTool on your computer, GeneTool is capable of transferring translated sequences or the results from remote BLAST protein searches to PepTool. Choose Sequence Editor, select Analyze and then the Translate option. Selecting Transfer will bring you into PepTool, for protein sequence analysis. 

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Can I transfer foreign sequence information into GeneTool's sequence editor?
GeneTool is capable of importing a number of different types of foreign sequence files into the Sequence Editor. These include GenBank, DDBJ, EMBL, dbEST, dbSTS, dbGSS, FASTA, ABI Chromatogram, SCF Chromatogram, and raw sequence files. If a file specifies sequence features, the Sequence Editor will automatically assign a unique colour and button to each feature, and it will highlight the relevant sequence segments. Sequence information is also extracted from foreign sequence files and may be viewed by clicking the "i" button in the Sequence Editor. 

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Can I export files in foreign formats from GeneTool?
Yes, GeneTool is capable of exporting DNA sequences to a foreign sequence file. Currently, GeneTool can export in GenBank, DDBJ, EMBL, and FASTA formats. 

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How much memory do I need to run GeneTool?
GeneTool requires a minimum of 32MB of physical RAM to run the program. 64MB RAM is recommended. 

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When performing a search or alignment on the Macintosh, I get an error message during the procedure saying that I have insufficient memory.
You need to reduce the buffer size used by GeneTool. To do this, open the Preferences window from the GeneTool Launcher, under the Advanced category, select the Memory page. Click on the Restore Default Settings button and the buffer size will be recalculated. After clicking on the OK button, you will be able to perform this analysis. 

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When I do a Fast Alignment Search or a Find Repeats/Vectors Search, I get an error message that my buffer size is too small.
The sequence you are searching against requires more memory than GeneTool is configured to use. To allow the search to use more memory, open the Preferences window from the GeneTool Launcher, under the Advanced category, select the Memory page. Enter a larger number in the Buffer Size field and click OK. It is not recommended that you set this number larger than the amount of physical memory available to GeneTool, because setting a buffer size that is too large for your computer may cause the search to run very slowly or not at all. You may find that you are not able to run these searches on sequences of this length with the amount of memory your machine has.  On a Macintosh, you may also need to increase the Prefered Size of the GeneTool:program:GeneTool application using Get Info from the Finder file menu.

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I have installed more memory on my computer but GeneTool continues to give me errors that my buffer size is too small and I am not able to run larger alignments.
You need to recalculate the buffer size. Open the Preferences window from the GeneTool Launcher, under the Advanced category, select the Memory page. Click on the Restore Default Settings button, and the buffer size will be recalculated. After clicking on the OK button, GeneTool will be able to use the new memory.  On a Macintosh, you may also need to increase the Prefered Size of the GeneTool:program:GeneTool application using Get Info from the Finder file menu.

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How do I do a database search against a sequence in GeneTool?
Once you have a sequence open in a Sequence Editor, select Fast Alignment Search or Remote BLAST Search from the Analyze menu.  The Sequence Editor's Fast Alignment Search command searches databases for sequences with matching or similar regions to the currently loaded sequence or selected sequence fragment using the local machine. BLAST searches may be used to identify sequences of common ancestry or of similar function to the currently loaded sequence or selected sequence fragment by connecting to a BLAST server.

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How do I integrate my personal database with GeneTool?
You can install non-BioTools database files using the Add File button in the Browse All Databases dialog box. GeneTool recognizes the following database formats: GenBank, DDBJ, EMBL, and FASTA. The Browse All Databases command can be found in the Maintenance menu of the Database Search window. 
Browse help to obtain a better understanding of GeneTool's database utilities:

  1. Click on the Help button in the GeneTool Launcher.
  2. In the Help Viewer, click on the Home button.
  3. Select the Index link in the left frame of the Help Viewer.
  4. Select the Database Maintenance link in the left frame of the Help Viewer.
  5. Select the Browsing Databases link in the left frame of the Help Viewer. Instructions for using the Browse All Databases command appears in the right frame of the Help Viewer. Non-BTI database files may be installed by using the Add File button.
  6. Follow the various links in the help page for browsing databases for more information about GeneTool's databases. (Note: you can navigate through the various help pages by clicking on the arrow keys in the Help Viewer.) 
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